AFAIK: If you don't compile EQEmu for 64-bit Windows then you will need the 32-bit version of the Perl and MySQL libraries.
The 32-bit version of EQEmu server will still be able to connect to a 64-bit version of the MySQL server, it just can't load 64-bit libraries.
Unless you are connecting to the database remotely then EQEmu should be connecting via localhost; you do not need to forward port 3306. If you mean "open" as in you can connect to it locally then that just means the MySQL server is running.
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